7BBF
Crystal structure of ubiquitin charged Ube2N (Ube2N~Ub) in complex with Ube2V2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 32 |
Unit cell lengths | 145.840, 145.840, 49.230 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.740 - 2.540 |
R-factor | 0.2081 |
Rwork | 0.206 |
R-free | 0.25340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5S |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.740 | 2.630 |
High resolution limit [Å] | 2.540 | 2.540 |
Rmerge | 0.054 | 1.347 |
Number of reflections | 38540 | 3845 |
<I/σ(I)> | 15.81 | |
Completeness [%] | 99.9 | |
Redundancy | 5.3 | |
CC(1/2) | 0.999 | 0.497 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1 M bicine Trizma base pH 8.5, 12.5% PEG 1000, 12.5% PEG 3350, 12.5% MPD, 0.2 % of each Anesthetic alkaloids (lidocaine HCl H2O, procaine HCl, proparacaine HCl, tetracaine HCl) |