7BAG

C3b in complex with CP40

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9677
Spacegroup name	P 1 21 1
Unit cell lengths	101.508, 90.528, 140.093
Unit cell angles	90.00, 108.84, 90.00

Refinement procedure

Resolution	47.820 - 2.000
R-factor	0.1957
Rwork	0.194
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wii
Data reduction software	XDS
Data scaling software	Aimless (0.3.8)
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.030	48.030	2.030
High resolution limit [Å]	2.000	10.950	2.000
Rmerge	0.097	0.049	1.961
Rmeas	0.111	0.056	2.243
Rpim	0.054	0.028	1.077
Total number of observations	666117	3928	33091
Number of reflections	159033	1025	7747
<I/σ(I)>	8.7	26.2	0.8
Completeness [%]	98.3	97.5	97.4
Redundancy	4.2	3.8	4.3
CC(1/2)	0.996	0.994	0.373

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	291	6.66% w/v polyethylene glycol 8000, 66.6 mM sodium chloride and 33.3 mM disodium phosphate/citric acid pH 4.0