7B91

Structure of a minimal SF3B core in complex with pladienolide D (form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.023
Spacegroup name	P 21 21 21
Unit cell lengths	106.075, 109.799, 251.936
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.330 - 3.000
R-factor	0.2124
Rwork	0.210
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ife 6en4
RMSD bond length	0.003
RMSD bond angle	0.565
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.330	2.870
High resolution limit [Å]	2.810	2.810
Rmerge	0.091	2.635
Rmeas	0.095	2.747
Rpim	0.026	0.767
Number of reflections	72883	4415
<I/σ(I)>	16.5	1
Completeness [%]	100.0	99.6
Redundancy	12.9	12.6
CC(1/2)	0.999	0.474

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.1M Bis-Tris Propane pH 7.63, PEG-3350 11%, Glycerol 15%, KSCN 0.2M