7B7J

EccD5 ubiqutin like domain from Mycobacterium xenopi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97
Spacegroup name	P 43 21 2
Unit cell lengths	43.979, 43.979, 85.146
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.610 - 1.660
R-factor	0.183
Rwork	0.181
R-free	0.21630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kv2
RMSD bond length	0.032
RMSD bond angle	3.305
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.570	1.690
High resolution limit [Å]	1.660	1.660
Rmerge	0.049	1.522
Number of reflections	10396	510
<I/σ(I)>	13.7
Completeness [%]	99.2	99.4
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.06 M MgCl2, CaCl2, 0.1 M Tris:bicine pH 8.5, 10 % OEG 20k, 20 % PEG MME 550