7B7J
EccD5 ubiqutin like domain from Mycobacterium xenopi
Summary for 7B7J
| Entry DOI | 10.2210/pdb7b7j/pdb |
| Descriptor | Secretion protein Snm4 (2 entities in total) |
| Functional Keywords | ubiqutin-like cytosolic esx secretion, membrane protein |
| Biological source | Mycobacterium xenopi |
| Total number of polymer chains | 1 |
| Total formula weight | 14017.20 |
| Authors | Beckham, K.S.,Wilmanns, M. (deposition date: 2020-12-10, release date: 2021-07-07, Last modification date: 2024-01-31) |
| Primary citation | Beckham, K.S.H.,Ritter, C.,Chojnowski, G.,Ziemianowicz, D.S.,Mullapudi, E.,Rettel, M.,Savitski, M.M.,Mortensen, S.A.,Kosinski, J.,Wilmanns, M. Structure of the mycobacterial ESX-5 type VII secretion system pore complex. Sci Adv, 7:-, 2021 Cited by PubMed Abstract: The ESX-5 type VII secretion system is a membrane-spanning protein complex key to the virulence of mycobacterial pathogens. However, the overall architecture of the fully assembled translocation machinery and the composition of the central secretion pore have remained unknown. Here, we present the high-resolution structure of the 2.1-megadalton ESX-5 core complex. Our structure captured a dynamic, secretion-competent conformation of the pore within a well-defined transmembrane section, sandwiched between two flexible protein layers at the cytosolic entrance and the periplasmic exit. We propose that this flexibility endows the ESX-5 machinery with large conformational plasticity required to accommodate targeted protein secretion. Compared to known secretion systems, a highly dynamic state of the pore may represent a fundamental principle of bacterial secretion machineries. PubMed: 34172453DOI: 10.1126/sciadv.abg9923 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.66 Å) |
Structure validation
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