7B26

CirpA1 in complex with pseudo-monomeric Properdin lacking TSR2-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.915890
Spacegroup name	P 21 21 2
Unit cell lengths	166.310, 54.512, 70.581
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.710 - 3.400
R-factor	0.2315
Rwork	0.229
R-free	0.27410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CirpA1 6s08
RMSD bond length	0.004
RMSD bond angle	0.874
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18rc4_3812)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.155	3.277
High resolution limit [Å]	2.842	2.842
Rmerge		1.820
Number of reflections	7444	372
<I/σ(I)>	6.1
Completeness [%]	89.4
Redundancy	10.7
CC(1/2)	0.994	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M Potassium thiocyanate, 0.1 M Tris, pH 7.5, 18 % w/v PEG 5000 MME