7ARW
Structure of human ARH3 E41A bound to alpha-NAD+ and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.746, 66.112, 70.055 |
| Unit cell angles | 115.80, 94.98, 104.07 |
Refinement procedure
| Resolution | 36.010 - 1.310 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.18200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6d3a |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.520 | 1.330 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Rmeas | 0.077 | 1.089 |
| Rpim | 0.029 | 0.417 |
| Total number of observations | 1042072 | 50580 |
| Number of reflections | 157336 | 7642 |
| <I/σ(I)> | 9.8 | 0.6 |
| Completeness [%] | 95.6 | 92.8 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 292 | 100 mM ammonium acetate (pH 4.5) and 9% (w/v) PEG10000 |
