7AMK

Zebrafish RET Cadherin Like Domains 1 to 4.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9787
Spacegroup name	P 1
Unit cell lengths	51.167, 70.499, 105.438
Unit cell angles	105.41, 100.93, 100.25

Refinement procedure

Resolution	65.960 - 2.200
R-factor	0.2273
Rwork	0.225
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x2u
RMSD bond length	0.008
RMSD bond angle	0.888
Data reduction software	DIALS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (dev_3965)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.960	2.190
High resolution limit [Å]	2.080	2.080
Rmerge	0.080	1.186
Rmeas	0.095	1.416
Rpim	0.051	0.765
Number of reflections	79868	11549
<I/σ(I)>	6.2	1.4
Completeness [%]	98.2	97.2
Redundancy	3.4	3.3
CC(1/2)	0.996	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	50 mM MES (pH 6.2), 31.5 % PEG MME 350 (v/v)