7ALQ
human GCH-GFRP inhibitory complex 7-deaza-GTP bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-21 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99988 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.104, 185.418, 184.231 |
| Unit cell angles | 90.00, 97.94, 90.00 |
Refinement procedure
| Resolution | 182.470 - 2.205 |
| R-factor | 0.1795 |
| Rwork | 0.177 |
| R-free | 0.22190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wpl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7 (18-SEP-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 182.470 | 2.464 |
| High resolution limit [Å] | 2.205 | 2.205 |
| Rmerge | 0.113 | 0.902 |
| Number of reflections | 223947 | 11196 |
| <I/σ(I)> | 9.3 | 1.6 |
| Completeness [%] | 93.9 | 69 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 0.2 M Sodium malonate pH 7.0; 20% w/v PEG3350 (Index H03) |
