7ALA
human GCH-GFRP inhibitory complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.999880 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.745, 114.013, 113.830 |
| Unit cell angles | 90.00, 130.34, 90.00 |
Refinement procedure
| Resolution | 39.740 - 1.846 |
| R-factor | 0.1867 |
| Rwork | 0.185 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wpl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.020 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC (1.1.7) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.054 | 2.051 |
| High resolution limit [Å] | 1.846 | 1.846 |
| Rmerge | 0.069 | 0.634 |
| Number of reflections | 67925 | 3398 |
| <I/σ(I)> | 11.3 | 1.7 |
| Completeness [%] | 94.5 | 81.1 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | GOL-P4K; 0.1 M MB3 pH 8.5; 0.12 M Monosaccharides (Morpheus F11) |
