7AJ6

LN02 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980107
Spacegroup name	C 1 2 1
Unit cell lengths	76.019, 66.448, 97.559
Unit cell angles	90.00, 95.92, 90.00

Refinement procedure

Resolution	48.520 - 1.900
R-factor	0.1783
Rwork	0.176
R-free	0.21420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j6e 4yka 4npy 6azz
RMSD bond length	0.009
RMSD bond angle	1.107
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.520	1.968
High resolution limit [Å]	1.900	1.900
Number of reflections	37744	3698
<I/σ(I)>	15.38
Completeness [%]	98.7
Redundancy	7
CC(1/2)	0.999	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M HEPES pH 7 30%(v/v) jeffamine M-600 reagent pH 7.0