7AEW
14-3-3 sigma bound to bis-phosphorylated aminopeptidase N (APN, CD13) via canonical and non-canonical binding motifs
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-29 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.911650 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.210, 112.060, 62.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.516 - 1.200 |
| Rwork | 0.162 |
| R-free | 0.18360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mhr |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.876 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.520 | 1.300 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 86519 | 16344 |
| <I/σ(I)> | 19.82 | |
| Completeness [%] | 95.8 | |
| Redundancy | 13.4 | |
| CC(1/2) | 1.000 | 0.854 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M HEPES pH 7.7 0.19 M CaCl2 5% glycerol 27 % PEG400 |
