7A59

Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Orthorhombic Crystal Form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.044
Spacegroup name	C 2 2 21
Unit cell lengths	69.961, 216.083, 274.283
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.910 - 2.197
Rwork	0.161
R-free	0.19420
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.827
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	AutoSol
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.370	49.370	2.230
High resolution limit [Å]	2.197	12.030	2.197
Rmerge	0.071	0.032	1.167
Rmeas	0.076	0.035	1.256
Rpim	0.028	0.013	0.459
Number of reflections	105875	732	4975
<I/σ(I)>	15.4
Completeness [%]	99.8	98.4	95.8
Redundancy	7.1	6.7	7.2
CC(1/2)	0.999	0.999	0.674

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291.15	0.75 uL of 12 mg/mL protein in 20 mM Tris-Cl pH 8.0 and 150 mM NaCl were added to 0.50 uL of reservoir solution containing 0.1 M MES pH 6.5, 0.1 M MgCl2 and 30% (v/v) PEG 400