7A3O
Crystal structure of dengue 1 virus envelope glycoprotein in complex with the scFv fragment of the broadly neutralizing human antibody EDE1 C10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 135.555, 135.555, 160.268 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.760 - 2.800 |
R-factor | 0.2207 |
Rwork | 0.218 |
R-free | 0.26780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ut9 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 21987 | 3110 |
<I/σ(I)> | 14.5 | |
Completeness [%] | 99.6 | 98.8 |
Redundancy | 8.2 | |
CC(1/2) | 0.999 | 0.512 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 291 | 50 mM Tris pH 8.5, 25% PEG 3,350, 200 mM (NH4)2SO4, 1.2% myo-inositol |