7A3N

Crystal structure of Zika virus envelope glycoprotein in complex with the Fab fragment of the broadly neutralizing human antibody EDE1 C10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9870
Spacegroup name	C 2 2 21
Unit cell lengths	53.964, 168.610, 220.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.910 - 2.100
R-factor	0.2045
Rwork	0.203
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lbv 4ut9
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.580	2.040
High resolution limit [Å]	1.990	1.990
Rmerge	0.664
Number of reflections	68764	3874
<I/σ(I)>	5.3
Completeness [%]	98.9
Redundancy	7.8
CC(1/2)	0.954	0.111

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	100 mM Imidazole (pH 8), 10% PEG 8,000, 200 mM CaCl2