6ZZ4
Crystal structure of the PTPN2 C216G mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97934 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 142.890, 55.980, 101.420 |
Unit cell angles | 90.00, 120.74, 90.00 |
Refinement procedure
Resolution | 48.710 - 2.430 |
R-factor | 0.2008 |
Rwork | 0.198 |
R-free | 0.25260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l8k |
RMSD bond length | 0.008 |
RMSD bond angle | 1.037 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.710 | 2.520 |
High resolution limit [Å] | 2.430 | 2.430 |
Rmerge | 0.118 | 0.640 |
Rmeas | 0.128 | 0.694 |
Rpim | 0.048 | 0.263 |
Number of reflections | 25798 | 2471 |
<I/σ(I)> | 10.97 | 2.38 |
Completeness [%] | 98.2 | 94.26 |
Redundancy | 6.9 | 6.8 |
CC(1/2) | 0.996 | 0.872 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 0.1 M Tris HCl pH 8.5, 2 M ammonium phosphate |