6ZWK
Crystal structure of the phosphorylated C-terminal tail of histone H2AX in complex with a specific nanobody (C6 gammaXbody)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-08 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98 |
Spacegroup name | P 31 |
Unit cell lengths | 87.412, 87.412, 105.409 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 61.490 - 1.550 |
R-factor | 0.1508 |
Rwork | 0.149 |
R-free | 0.18440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lz0 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (dev_3915) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 61.490 | 61.490 | 1.580 |
High resolution limit [Å] | 1.550 | 8.490 | 1.550 |
Rmerge | 0.091 | 0.042 | 2.268 |
Rmeas | 0.095 | 0.045 | 2.384 |
Rpim | 0.029 | 0.014 | 0.732 |
Total number of observations | 1376764 | 7784 | 67802 |
Number of reflections | 130679 | 795 | 6425 |
<I/σ(I)> | 12.2 | 37.1 | 1 |
Completeness [%] | 99.9 | 99.7 | 99.9 |
Redundancy | 10.5 | 9.8 | 10.6 |
CC(1/2) | 0.999 | 0.999 | 0.635 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% PEG 3350, 0.2M Sodium acetate |