6ZWC
Z-SBTub2 photoswitch bound to tubulin-DARPin D1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.900, 91.850, 82.810 |
| Unit cell angles | 90.00, 96.58, 90.00 |
Refinement procedure
| Resolution | 45.185 - 2.040 |
| R-factor | 0.2268 |
| Rwork | 0.225 |
| R-free | 0.28150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nm5 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.185 | 2.327 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.134 | 1.124 |
| Rmeas | 0.154 | 1.277 |
| Rpim | 0.074 | 0.591 |
| Number of reflections | 41022 | 2024 |
| <I/σ(I)> | 7.1 | 1.6 |
| Completeness [%] | 59.5 | 9.3 |
| Redundancy | 4.1 | 4.7 |
| CC(1/2) | 0.992 | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3000, ammonium sulfate, bis-tris methane |
