6ZVO

Crystal structure of unliganded MLKL executioner domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.99988
Spacegroup name	P 1 21 1
Unit cell lengths	56.057, 53.501, 70.912
Unit cell angles	90.00, 98.36, 90.00

Refinement procedure

Resolution	70.160 - 1.371
R-factor	0.1918
Rwork	0.190
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4btf
RMSD bond length	0.010
RMSD bond angle	0.940
Data reduction software	AutoProcess (1.1.7)
Data scaling software	AutoProcess
Phasing software	MOLREP
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.158	1.483
High resolution limit [Å]	1.375	1.375
Number of reflections	65990	3300
<I/σ(I)>	18.3
Completeness [%]	94.4
Redundancy	6.5
CC(1/2)	0.999	0.518

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	31 % Polyethylene glycol monomethyl ether 2,000, 0.15 M Potassium bromide, 0.1 M TRIS, pH 9