6ZN6

Protein polybromo-1 (PB1 BD2) Bound To MW278

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-08
Detector	PSI PILATUS 6M
Wavelength(s)	0.99999
Spacegroup name	C 2 2 21
Unit cell lengths	70.525, 73.193, 105.024
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.600 - 2.020
R-factor	0.221
Rwork	0.218
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hmf
RMSD bond length	0.008
RMSD bond angle	0.867
Data reduction software	xia2
Data scaling software	Aimless (7.0.078)
Phasing software	PHASER (7.0.078)
Refinement software	REFMAC (7.0.078)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.600	2.090
High resolution limit [Å]	2.020	2.020
Number of reflections	18158	1777
<I/σ(I)>	7.9
Completeness [%]	99.9
Redundancy	7.4
CC(1/2)	0.991	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.2 M NaCl 25% (w/v) PEG3350 0.1 M bis-tris pH 5.5