6ZMK

Crystal structure of human GFAT-1 L405R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-02-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	152.945, 152.945, 166.213
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.310 - 2.382
R-factor	0.1932
Rwork	0.193
R-free	0.21060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6r4e
RMSD bond length	0.002
RMSD bond angle	0.452
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	XDS (Mar 15, 2019)
Phasing software	PHENIX (dev_2499)
Refinement software	PHENIX (dev_2499)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.310	2.470
High resolution limit [Å]	2.380	2.380
Number of reflections	78975	7737
<I/σ(I)>	20.15
Completeness [%]	99.9
Redundancy	13.4
CC(1/2)	0.999	0.501

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M Bis tris propane pH 8.5, 0.3 M Potassium sodium tartrate, 20 % PEG3350