6ZEG

Structure of PP1-IRSp53 chimera [PP1(7-304) + linker (G/S)x9 + IRSp53(449-465)] bound to Phactr1 (516-580)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.8
Spacegroup name	P 1 21 1
Unit cell lengths	48.616, 122.394, 69.097
Unit cell angles	90.00, 92.22, 90.00

Refinement procedure

Resolution	37.180 - 1.090
R-factor	0.1206
Rwork	0.119
R-free	0.14030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mov
RMSD bond length	0.010
RMSD bond angle	1.175
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.180	1.130
High resolution limit [Å]	1.090	1.090
Rmerge	0.018	0.440
Rmeas	0.026	0.623
Rpim	0.018	0.440
Number of reflections	332381	62356
<I/σ(I)>	15.41	1.88
Completeness [%]	99.3
Redundancy	2
CC(1/2)	1.000	0.654

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	20% (w/v) polyethylene glycol 3350, 0.2 M KSCN and 0.1 M BIS-Tris propane pH 8.5