6ZEE

Structure of PP1(7-300) bound to Phactr1 (507-580) at pH8.4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97
Spacegroup name	P 65
Unit cell lengths	137.695, 137.695, 238.793
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	119.250 - 1.900
R-factor	0.2373
Rwork	0.236
R-free	0.26830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mov
RMSD bond length	0.003
RMSD bond angle	0.610
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	238.790	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.144	2.140
Rmeas	0.156
Rpim	0.045	0.750
Number of reflections	2420639	200842
<I/σ(I)>	7.8	1.1
Completeness [%]	100.0	100
Redundancy	12.1	10
CC(1/2)	0.999	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	293	7.5% PEG 3350, 0.2M MgSO4