6ZD5
Crystal structure of YTHDC1 S378A mutant complex with m6A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-17 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.820, 103.400, 42.490 |
Unit cell angles | 90.00, 105.77, 90.00 |
Refinement procedure
Resolution | 40.890 - 2.300 |
R-factor | 0.1883 |
Rwork | 0.186 |
R-free | 0.23310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r3h |
RMSD bond length | 0.008 |
RMSD bond angle | 0.941 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.890 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 14545 | 2296 |
<I/σ(I)> | 25.77 | |
Completeness [%] | 99.8 | |
Redundancy | 4.5 | |
CC(1/2) | 0.998 | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG3350, 0.2M Ammonium Sulphate, 0.1M bis-tris, pH 6.5 |