6ZBZ
Small-molecule inhibitors of the PDZ domain of Dishevelled proteins interrupt Wnt signalling
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2009-05-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.91841 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.837, 70.059, 87.182 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.590 - 1.600 |
R-factor | 0.1771 |
Rwork | 0.175 |
R-free | 0.22280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2f0a |
RMSD bond length | 0.001 |
RMSD bond angle | 0.999 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.590 | 1.640 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmeas | 0.004 | 0.080 |
Number of reflections | 46555 | 3405 |
<I/σ(I)> | 23.6 | |
Completeness [%] | 99.7 | |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG 8000, 0.1 M MES pH 5.5 |