6Z4A
Structure of the human SAS-6 N-terminal domain, F131E mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 59.590, 65.130, 38.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.980 - 1.460 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y3v |
RMSD bond length | 0.009 |
RMSD bond angle | 1.100 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.980 | 1.490 |
High resolution limit [Å] | 1.460 | 1.460 |
Rmerge | 0.060 | 1.118 |
Rmeas | 0.066 | 1.238 |
Rpim | 0.027 | 0.526 |
Number of reflections | 26380 | 1273 |
<I/σ(I)> | 14.6 | 1.4 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 6.1 | 5.4 |
CC(1/2) | 0.999 | 0.566 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1 M HEPES 30% w/v PEG 6000 Cryoprotected by addition of 20% v/v glycerol |