2Y3V
N-terminal head domain of Danio rerio SAS-6
Summary for 2Y3V
| Entry DOI | 10.2210/pdb2y3v/pdb |
| Related | 2Y3W |
| Descriptor | SPINDLE ASSEMBLY ABNORMAL PROTEIN 6 HOMOLOG (2 entities in total) |
| Functional Keywords | structural protein, cytoskeleton, basal body, centriole, cartwheel, cartwheel hub |
| Biological source | DANIO RERIO (ZEBRAFISH) |
| Cellular location | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome : Q7ZVT3 |
| Total number of polymer chains | 4 |
| Total formula weight | 73095.46 |
| Authors | van Breugel, M. (deposition date: 2010-12-27, release date: 2011-02-09, Last modification date: 2024-05-01) |
| Primary citation | van Breugel, M.,Hirono, M.,Andreeva, A.,Yanagisawa, H.A.,Yamaguchi, S.,Nakazawa, Y.,Morgner, N.,Petrovich, M.,Ebong, I.O.,Robinson, C.V.,Johnson, C.M.,Veprintsev, D.,Zuber, B. Structures of SAS-6 suggest its organization in centrioles. Science, 331:1196-1199, 2011 Cited by PubMed Abstract: Centrioles are cylindrical, ninefold symmetrical structures with peripheral triplet microtubules strictly required to template cilia and flagella. The highly conserved protein SAS-6 constitutes the center of the cartwheel assembly that scaffolds centrioles early in their biogenesis. We determined the x-ray structure of the amino-terminal domain of SAS-6 from zebrafish, and we show that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers. Point mutations are consistent with the notion that centriole formation in vivo depends on the interactions that define the self-assemblies observed here. Thus, these interactions are probably essential to the structural organization of cartwheel centers. PubMed: 21273447DOI: 10.1126/science.1199325 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.92 Å) |
Structure validation
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