6Z49
Crystal structure of deubiquitinase Mindy2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.07252 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.115, 120.420, 78.378 |
Unit cell angles | 90.00, 92.94, 90.00 |
Refinement procedure
Resolution | 47.720 - 2.000 |
R-factor | 0.2172 |
Rwork | 0.215 |
R-free | 0.25110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jkn |
RMSD bond length | 0.008 |
RMSD bond angle | 1.567 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MoRDa |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.720 | 2.040 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.052 | 0.673 |
Rmeas | 0.059 | 0.773 |
Rpim | 0.028 | 0.376 |
Total number of observations | 344672 | |
Number of reflections | 83541 | 4588 |
<I/σ(I)> | 13.9 | |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 4.1 | 4.1 |
CC(1/2) | 0.999 | 0.814 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293.15 | 0.1 M Bis-Tris pH 6.0, 0.2 M Magnesium chloride and 25% PEG 3350 |