6Z3L
Repulsive Guidance Molecule C (RGMC, Hemojuvelin, HJV, HFE2) in complex with Growth Differentiation Factor 5 (GDF5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 98.820, 98.820, 99.230 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 85.580 - 2.513 |
R-factor | 0.2395 |
Rwork | 0.238 |
R-free | 0.26730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ui1 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.386 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13rc2_2986: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.580 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.120 | |
Rpim | 1.125 | |
Number of reflections | 10324 | 721 |
<I/σ(I)> | 23.9 | 0.7 |
Completeness [%] | 99.0 | |
Redundancy | 47.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 1 M LiCl, 0.1 M citric acid pH 4.0, 8% gamma-butyrolactone. |