6Z30
Human cation-independent mannose 6-phosphate/ IGF2 receptor domains 9-10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-10-12 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.360, 55.830, 132.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.460 - 1.500 |
| R-factor | 0.1977 |
| Rwork | 0.199 |
| R-free | 0.22840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Homology models |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.923 |
| Data reduction software | xia2 (0.5.902) |
| Data scaling software | DIALS (1.14) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.460 | 1.810 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 48880 | 3040 |
| <I/σ(I)> | 11.12 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.9 | 12.4 |
| CC(1/2) | 0.999 | 0.744 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 0.1 M SPG buffer (succinic acid, sodium dihydrogen phosphate monohydrate, glycine) pH 9, 25 % PEG 1500 |
