6YPC

Crystal structure of the kinetochore subunits H/I/K/T/W penta-complex from S. cerevisiae at 2.9 angstroms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9465
Spacegroup name	I 41 2 2
Unit cell lengths	132.577, 132.577, 241.676
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.850 - 2.900
R-factor	0.2312
Rwork	0.228
R-free	0.28290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5Z08 and 3B0D
RMSD bond length	0.010
RMSD bond angle	1.263
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.070	3.070
High resolution limit [Å]	2.900	2.900
Rmerge	0.368
Rpim	0.047	1.826
Number of reflections	24344	3860
<I/σ(I)>	10
Completeness [%]	100.0
Redundancy	63.3
CC(1/2)	0.999	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1 M NaH2PO4 and 0.38M K2HPO4 with protein at 5.5 mg/ml