6YMF
Crystal structure of serine hydroxymethyltransferase from Aphanothece halophytica in the PLP-Serine external aldimine state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 93.888, 93.888, 134.683 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.890 - 1.630 |
| R-factor | 0.1067 |
| Rwork | 0.106 |
| R-free | 0.12980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ymd |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.813 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.890 | 1.730 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmeas | 0.045 | 1.098 |
| Number of reflections | 86052 | 13719 |
| <I/σ(I)> | 29.5 | 2.1 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 11.3 | 11.3 |
| CC(1/2) | 1.000 | 0.847 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292 | 0.05 M citric acid, 0.05 M BIS-TRIS propane at pH 5.0 and 16% polyethylene glycol 3350 cocrystallized with 50 mM L-serine |
