6YLV
Translation initiation factor 4E in complex with 4-Cl-Bn7GpppG mRNA 5' cap analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 1 |
Unit cell lengths | 38.350, 38.390, 148.080 |
Unit cell angles | 85.28, 88.42, 77.23 |
Refinement procedure
Resolution | 49.190 - 2.660 |
R-factor | 0.203474516796 |
Rwork | 0.201 |
R-free | 0.25527 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l8b |
RMSD bond length | 0.006 |
RMSD bond angle | 0.747 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.755 |
High resolution limit [Å] | 2.660 | 2.660 |
Rmerge | 0.112 | 0.571 |
Rmeas | 0.126 | 0.680 |
Rpim | 0.056 | 0.361 |
Number of reflections | 22090 | 1453 |
<I/σ(I)> | 10.32 | 2.04 |
Completeness [%] | 93.6 | 60.42 |
Redundancy | 4.7 | 2.8 |
CC(1/2) | 0.991 | 0.764 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1 M Bicine pH 8.5, 0.2 M sodium formate, 19 % w/v PEGMME 5000 |