6YK5
Structure of the AMPA receptor GluA2o ligand-binding domain (S1S2J) in complex with the compound (S)-1-(2'-Amino-2'-carboxyethyl)-5,7-dihydrofuro[3,4-d]- pyrimidine-2,4(1H,3H)-dione at resolution 1.15A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 59.075, 96.331, 48.522 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.778 - 1.150 |
R-factor | 0.1108 |
Rwork | 0.110 |
R-free | 0.13010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1syh |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.9) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.212 | 26.778 | 1.210 |
High resolution limit [Å] | 1.150 | 3.640 | 1.150 |
Rmerge | 0.035 | 0.385 | |
Rmeas | 0.069 | 0.040 | 0.452 |
Rpim | 0.032 | 0.019 | 0.231 |
Total number of observations | 450981 | 15244 | 44337 |
Number of reflections | 97655 | 3359 | 12982 |
<I/σ(I)> | 14.2 | 35.7 | 3.2 |
Completeness [%] | 98.6 | 99 | 90.7 |
Redundancy | 4.6 | 4.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 279 | PEG4000, lithium sulfate, acetate |