6YK4
Structure of the AMPA receptor GluA2o ligand-binding domain (S1S2J) in complex with the compound ( S) - 1- [2'-Amino-2'-carboxyethyl]-6-methyl-5 ,7- dihydropyrrolo[3,4-d]pyrimidin-2,4(1H,3H)-dione at resolution 1.00A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 103.8 |
Detector technology | CCD |
Collection date | 2013-06-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.90000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 59.213, 96.542, 48.543 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.543 - 0.999 |
R-factor | 0.1072 |
Rwork | 0.106 |
R-free | 0.11990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1syh |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.558 | 48.543 | 1.050 |
High resolution limit [Å] | 0.999 | 3.160 | 1.000 |
Rmerge | 0.030 | 0.373 | |
Rmeas | 0.051 | 0.032 | 0.424 |
Rpim | 0.016 | 0.010 | 0.198 |
Total number of observations | 1041985 | 52021 | 95422 |
Number of reflections | 150574 | 5123 | 21664 |
<I/σ(I)> | 19.6 | 71.3 | 3.9 |
Completeness [%] | 99.9 | 99.9 | 99.3 |
Redundancy | 6.9 | 10.2 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 279 | 15.2% PEG4000, 0.3M lithium sulfate, 0.1M acetate |