6YJZ

Crystal structure of mouse pyridoxal kinase in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9724
Spacegroup name	C 1 2 1
Unit cell lengths	279.135, 53.433, 109.372
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.320 - 2.450
R-factor	0.2183
Rwork	0.216
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xyt
RMSD bond length	0.002
RMSD bond angle	0.525
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.320	2.530
High resolution limit [Å]	2.450	2.450
Rmerge	0.098	0.750
Rmeas	0.088	0.678
Rpim	0.093	0.700
Number of reflections	57180	5965
<I/σ(I)>	9.1	1.6
Completeness [%]	95.6	99.7
Redundancy	3.2	1.9
CC(1/2)	0.993	0.572

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.18-0.24 M sodium thiocyanate and 18-26% PEG 3350