6YJH
Crystal structure of Imidazole Glycerol Phosphate Dehydratase from Mycobacterium tuberculosis at 1.61 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-16 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 112.130, 112.130, 112.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.970 - 1.610 |
| R-factor | 0.1524 |
| Rwork | 0.151 |
| R-free | 0.17480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gqu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.795 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.700 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.099 | 0.380 |
| Rmeas | 0.100 | 0.428 |
| Number of reflections | 31512 | 4235 |
| <I/σ(I)> | 5.9569 | |
| Completeness [%] | 99.1 | 93.86 |
| Redundancy | 27.56 | 4.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 293 | 30% PEG1500, 0.2 M sodium citrate tribasic dehydrate, 0.1 M Tris pH 7.5, 5 mM MnCl2, 0.04% NaN3 |
