6YIF
Structure of Chromosomal Passenger Complex (CPC) bound to phosphorylated Histone 3 peptide at 1.8 A.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2017-10-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.96859 |
Spacegroup name | P 65 |
Unit cell lengths | 98.863, 98.863, 56.067 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.430 - 1.810 |
R-factor | 0.1828 |
Rwork | 0.181 |
R-free | 0.22270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qfa |
RMSD bond length | 0.011 |
RMSD bond angle | 1.149 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.430 | 1.860 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.048 | 0.953 |
Number of reflections | 28454 | 2126 |
<I/σ(I)> | 13.8 | 1.4 |
Completeness [%] | 99.4 | |
Redundancy | 4.4 | |
CC(1/2) | 0.997 | 0.544 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 291 | 20 % (w/v) PEG 4,000, 200 mM Lithium sulphate, 50 mM MES pH 6.5 |