6YIF

Structure of Chromosomal Passenger Complex (CPC) bound to phosphorylated Histone 3 peptide at 1.8 A.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	110
Detector technology	PIXEL
Collection date	2017-10-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96859
Spacegroup name	P 65
Unit cell lengths	98.863, 98.863, 56.067
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.430 - 1.810
R-factor	0.1828
Rwork	0.181
R-free	0.22270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qfa
RMSD bond length	0.011
RMSD bond angle	1.149
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.430	1.860
High resolution limit [Å]	1.810	1.810
Rmerge	0.048	0.953
Number of reflections	28454	2126
<I/σ(I)>	13.8	1.4
Completeness [%]	99.4
Redundancy	4.4
CC(1/2)	0.997	0.544

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	291	20 % (w/v) PEG 4,000, 200 mM Lithium sulphate, 50 mM MES pH 6.5