6YIE
Structure of a Borealin-INCENP-Survivin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2017-06-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97630 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.536, 78.824, 125.173 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.700 - 3.490 |
R-factor | 0.2714 |
Rwork | 0.269 |
R-free | 0.32430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qfa |
RMSD bond length | 0.004 |
RMSD bond angle | 0.810 |
Data reduction software | Aimless |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.820 | 3.830 |
High resolution limit [Å] | 3.490 | 3.490 |
Rmerge | 0.162 | 0.435 |
Number of reflections | 6947 | 1644 |
<I/σ(I)> | 3.5 | 1.7 |
Completeness [%] | 88.5 | |
Redundancy | 3.7 | |
CC(1/2) | 0.992 | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 298 | 5 % (w/v) PEG 3,350, 50 mM MES pH 6.0 |