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6YIE

Structure of a Borealin-INCENP-Survivin complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron sitePETRA III, EMBL c/o DESY
BeamlineP14 (MX2)
Temperature [K]110
Detector technologyPIXEL
Collection date2017-06-21
DetectorDECTRIS EIGER X 16M
Wavelength(s)0.97630
Spacegroup nameP 21 21 21
Unit cell lengths59.536, 78.824, 125.173
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution66.700 - 3.490
R-factor0.2714
Rwork0.269
R-free0.32430
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2qfa
RMSD bond length0.004
RMSD bond angle0.810
Data reduction softwareAimless
Data scaling softwareAimless
Phasing softwarePHASER
Refinement softwarePHENIX (1.16_3549)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]78.8203.830
High resolution limit [Å]3.4903.490
Rmerge0.1620.435
Number of reflections69471644
<I/σ(I)>3.51.7
Completeness [%]88.5
Redundancy3.7
CC(1/2)0.9920.902
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION62985 % (w/v) PEG 3,350, 50 mM MES pH 6.0

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