6YHR

Crystal structure of Werner syndrome helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-02
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.08
Spacegroup name	P 21 21 21
Unit cell lengths	54.595, 90.627, 138.233
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.120 - 2.200
R-factor	0.1987
Rwork	0.197
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wwy
RMSD bond length	0.002
RMSD bond angle	0.537
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.000	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.130	2.300
Rpim	0.060	1.130
Number of reflections	35356	2943
<I/σ(I)>	5.3	1
Completeness [%]	98.8	96
Redundancy	5.5
CC(1/2)	0.990	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	1M Na Acetate, 0.1 M Cacodylate pH 6.5