6YF1
FKBP12 in complex with the BMP potentiator compound 8 at 1.12A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.041, 36.680, 28.777 |
| Unit cell angles | 90.00, 103.72, 90.00 |
Refinement procedure
| Resolution | 17.180 - 1.120 |
| R-factor | 0.1559 |
| Rwork | 0.155 |
| R-free | 0.16340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dh0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.403 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.180 | 1.150 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.038 | 0.388 |
| Rmeas | 0.046 | 0.477 |
| Number of reflections | 37030 | 2265 |
| <I/σ(I)> | 15.3 | 2.9 |
| Completeness [%] | 94.7 | 79.2 |
| Redundancy | 3.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2.2 M AmSO4, 0.2 M CdCl2 |
