6YE3
IL-2 in complex with a Fab fragment from UFKA-20
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-05 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 238.384, 92.547, 121.892 |
Unit cell angles | 90.00, 100.11, 90.00 |
Refinement procedure
Resolution | 49.400 - 2.890 |
R-factor | 0.2185 |
Rwork | 0.215 |
R-free | 0.28330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b6f |
RMSD bond length | 0.009 |
RMSD bond angle | 1.317 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.400 | 2.993 |
High resolution limit [Å] | 2.890 | 2.890 |
Number of reflections | 114189 | 5708 |
<I/σ(I)> | 5.74 | 0.38 |
Completeness [%] | 99.7 | 98.21 |
Redundancy | 6.9 | 6.6 |
CC(1/2) | 0.992 | 0.186 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10.86% (v/v) PEG 8000, 5.76% (v/v) ethylene glycol, 100 mM HEPES, pH 7.48 |