6Y6B
Crystal structure of human 14-3-3 gamma in complex with CaMKK2 14-3-3 binding motif Ser100 and 16-OMe-Fusicoccin H
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-24 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 226.266, 226.266, 72.993 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 17.750 - 3.080 |
R-factor | 0.244 |
Rwork | 0.242 |
R-free | 0.28180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b05 |
RMSD bond length | 0.001 |
RMSD bond angle | 0.357 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.990 | 3.260 |
High resolution limit [Å] | 3.080 | 3.080 |
Rmeas | 0.280 | 4.970 |
Number of reflections | 20904 | 3285 |
<I/σ(I)> | 11.53 | 0.69 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 21.72 | 22 |
CC(1/2) | 0.999 | 0.226 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | PEG400, HEPES, magnesium chloride, hexafluoropropanol |