6Y4P
Calmodulin N53I variant bound to cardiac ryanodine receptor (RyR2) calmodulin binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-11 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.590, 42.360, 90.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.180 - 2.133 |
R-factor | 0.188711047719 |
Rwork | 0.182 |
R-free | 0.24621 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bcx |
RMSD bond length | 0.008 |
RMSD bond angle | 0.735 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.180 | 2.209 |
High resolution limit [Å] | 2.133 | 2.133 |
Number of reflections | 8489 | 831 |
<I/σ(I)> | 9.45 | |
Completeness [%] | 99.8 | |
Redundancy | 5.9 | |
CC(1/2) | 0.993 | 0.946 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.1 M Sodium Acetate pH 4.70 and 23 % PEG 550 MME |