6Y09

Crystal structure of Atg16L in complex with GTP-bound Rab33B (Q92L)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97714
Spacegroup name	P 21 21 21
Unit cell lengths	56.950, 132.420, 155.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.168 - 2.400
R-factor	0.1937
Rwork	0.192
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3Q8T and 1Z06
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.170	2.486
High resolution limit [Å]	2.400	2.400
Number of reflections	46731	4618
<I/σ(I)>	17.05	2.67
Completeness [%]	99.9	99.87
Redundancy	12.9	13.6
CC(1/2)	0.871	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277.15	14% PEG3350, 0.2 M Potassium thiocyanate and 0.1 M Sodium potassium phosphate