6XXW
Structure of beta-D-Glucuronidase for Dictyoglomus thermophilum.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 79.130, 115.820, 130.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.834 - 1.851 |
R-factor | 0.1622 |
Rwork | 0.160 |
R-free | 0.20690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bhg |
Data reduction software | XDS (20180808) |
Data scaling software | XDS (20180808) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.834 | 33.834 | 1.960 |
High resolution limit [Å] | 1.850 | 5.520 | 1.850 |
Rmerge | 0.111 | 0.030 | 0.743 |
Rmeas | 0.125 | 0.034 | 0.837 |
Total number of observations | 235094 | ||
Number of reflections | 50245 | 1982 | 7994 |
<I/σ(I)> | 12.15 | 35.43 | 2.46 |
Completeness [%] | 97.7 | 94.4 | 97 |
Redundancy | 4.679 | 4.483 | 4.531 |
CC(1/2) | 0.997 | 0.999 | 0.709 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 20% w/v 2-Methylpentane-2,2-diol 100mM NaHEPES pH 7.5 100mM Citrate sodium |