6XXW

Structure of beta-D-Glucuronidase for Dictyoglomus thermophilum.

Summary for 6XXW

Entry DOI	10.2210/pdb6xxw/pdb
Descriptor	Beta-glucuronidase, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (5 entities in total)
Functional Keywords	glycoside hydrolase, glucuronide, hydrolase
Biological source	Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12)
Total number of polymer chains	1
Total formula weight	71197.18
Authors	Lafite, P.,Daniellou, R. (deposition date: 2020-01-28, release date: 2020-07-15, Last modification date: 2024-01-24)
Primary citation	Kurdziel, M.,Kopec, M.,Paris, A.,Lewinski, K.,Lafite, P.,Daniellou, R. Thioglycoligation of aromatic thiols using a natural glucuronide donor. Org.Biomol.Chem., 18:5582-5585, 2020 Cited by PubMed Abstract: The β-d-glucuronidase DtGlcA from Dictyoglomus thermophilum was engineered to generate an active thioglycoligase that is able to catalyse the formation of numerous S-glucuronides. Its X-ray structure analysis indicated the ability of the biocatalyst to bind aromatic thiol acceptors for S-glycosylation. Noteworthily, the DtGlcA mutant was found to be the first thioligase that is able to use a natural sugar donor different from the widely used synthetic para-nitrophenyl glycosides. PubMed: 32671369 DOI: 10.1039/d0ob00226g PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.851 Å)

Structure validation