6XXJ
Crystal Structure of Human Deoxyhypusine Synthase in complex with spermidine and NAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 105.486, 105.486, 160.831 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.240 - 1.410 |
R-factor | 0.1427 |
Rwork | 0.142 |
R-free | 0.15980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dhs |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (v1.17.1) |
Refinement software | PHENIX (v1.17.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.240 | 46.240 | 1.490 |
High resolution limit [Å] | 1.410 | 4.210 | 1.410 |
Rmerge | 0.088 | 0.034 | 2.437 |
Rmeas | 0.094 | 0.036 | 2.573 |
Total number of observations | 1946524 | ||
Number of reflections | 198798 | 7863 | 31696 |
<I/σ(I)> | 13.34 | 56.25 | 0.76 |
Completeness [%] | 99.8 | 99.8 | 99.4 |
Redundancy | 9.791 | 8.924 | 9.729 |
CC(1/2) | 0.999 | 0.999 | 0.548 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.025-0.125 mM carboxylic acid mix, 30-60% precipitant mix (MPD, PEG 1000, PEG 3350), 100 mM Tris-Bicine pH = 8.5 |