6XVX

X-ray structure obtained upon reaction of dirhodium tetraacetate with RNase A (high resolution)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.96
Spacegroup name	C 1 2 1
Unit cell lengths	100.430, 32.750, 72.690
Unit cell angles	90.00, 90.38, 90.00

Refinement procedure

Resolution	72.688 - 1.400
Rwork	0.188
R-free	0.23260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ot4
RMSD bond length	0.012
RMSD bond angle	1.799
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.690	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.066	0.259
Rmeas	0.080	0.327
Rpim	0.055	0.196
Number of reflections	43787	2120
<I/σ(I)>	8.5	2.4
Completeness [%]	93.4	91.3
Redundancy	2.6	2.2
CC(1/2)	0.990	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.1	298	22% PEG 4K 10 mM sodium citrate buffer pH 5.1