6XVX
X-ray structure obtained upon reaction of dirhodium tetraacetate with RNase A (high resolution)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.96 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.430, 32.750, 72.690 |
| Unit cell angles | 90.00, 90.38, 90.00 |
Refinement procedure
| Resolution | 72.688 - 1.400 |
| Rwork | 0.188 |
| R-free | 0.23260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ot4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.799 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.690 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.066 | 0.259 |
| Rmeas | 0.080 | 0.327 |
| Rpim | 0.055 | 0.196 |
| Number of reflections | 43787 | 2120 |
| <I/σ(I)> | 8.5 | 2.4 |
| Completeness [%] | 93.4 | 91.3 |
| Redundancy | 2.6 | 2.2 |
| CC(1/2) | 0.990 | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 298 | 22% PEG 4K 10 mM sodium citrate buffer pH 5.1 |
