6XVX

X-ray structure obtained upon reaction of dirhodium tetraacetate with RNase A (high resolution)

This is a non-PDB format compatible entry.

Summary for 6XVX

• Entry DOI: 10.2210/pdb6xvx/pdb
• Descriptor: Ribonuclease pancreatic, ACETATE ION, RHODIUM(III) ION, ... (4 entities in total)
• Functional Keywords: dirhodium, model protein, metalation, unknown function
• Biological source: Bos taurus (cattle)
• Total number of polymer chains: 2
• Total formula weight: 29243.65

Authors

Merlino, A.,Ferraro, G. (deposition date: 2020-01-22, release date: 2020-02-19, Last modification date: 2024-11-20)

Primary citation

Ferraro, G.,Pratesi, A.,Messori, L.,Merlino, A.
Protein interactions of dirhodium tetraacetate: a structural study.
Dalton Trans, 49:2412-2416, 2020

PubMed Abstract: The interactions between the cytotoxic paddlewheel dirhodium complex [Rh2(μ-O2CCH3)4] and the model protein bovine pancreatic ribonuclease (RNase A) were investigated by high-resolution mass spectrometry and X-ray crystallography. The results indicate that [Rh2(μ-O2CCH3)4] extensively reacts with RNase A. The metal compound binds the protein via coordination of the imidazole ring of a His side chain to one of its axial sites, while the dirhodium center and the acetato ligands remain unmodified. Data provide valuable information for the design of artificial dirhodium-containing metalloenzymes.

PubMed: 32022076
DOI: 10.1039/c9dt04819g

Experimental method

X-RAY DIFFRACTION (1.4 Å)